Composite

Part:BBa_K1462230:Design

Designed by: Chuyao Fan   Group: iGEM14_SCUT   (2014-10-09)

Gal1+Crt+cyc1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The n-butanol pathway in the cytoplasm is constructed as a control group to identify the effectiveness of the pathway directed to the mitochondria. Crt,originated from Clostridium beijerinckii,expresses crotonase, which converts HbCoA to CrCoA, the third step of the n-butanol pathway.This part is constructed in pUC57 individually at the beginning and it is assembled with Hbd(BBa_K1462220),Erg10 (BBa_K1462210) in plasmid 181 ,which is leucine auxotroph.

Figure 1. The n-butanol biosynthetic pathway. Enzymes are from these organisms: Erg10, S. cerevisiae; Hbd, Crt, AdhE2, Clostridium beijerinckii; Ccr, Streptomyces collinus.