Composite
Part:BBa_K1462230:Design
Designed by: Chuyao Fan Group: iGEM14_SCUT (2014-10-09)
Gal1+Crt+cyc1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The n-butanol pathway in the cytoplasm is constructed as a control group to identify the effectiveness of the pathway directed to the mitochondria. Crt,originated from Clostridium beijerinckii,expresses crotonase, which converts HbCoA to CrCoA, the third step of the n-butanol pathway.This part is constructed in pUC57 individually at the beginning and it is assembled with Hbd(BBa_K1462220),Erg10 (BBa_K1462210) in plasmid 181 ,which is leucine auxotroph.